Pif4 brassinosteroid

The plant hormones brassinosteroids (BR) promote hypocotyl elongation of Arabidopsis thaliana ( A. thaliana ) seedlings both under light and dark (etiolated) conditions. A common assay to determine if a mutant or transgenic line is affected in BR biosynthesis or response is a sensitivity assay to brassinazole (BRZ), an inhibitor of P450 cytochromes specific to BR biosynthesis.
Here we provide a protocol to compare BRZ sensitivity of different A. thaliana genotypes in terms of hypocotyl elongation (Bernardo-García et al. , 2014).

Pif4 brassinosteroid

pif4 brassinosteroid

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